mtt dye Search Results


mtt dye  (Promega)
90
Promega mtt dye
Bioactivity of (4 E )- R -dysidazirine carboxylic acid ( 1 ) and methyl ester 2. ( A ) Antiproliferative activity against human colon cancer cells (HCT116) measured by <t>MTT</t> assay at 48 h. Gatorbulin-1 was used as a positive control, tested at the same time (IC 50 0.80 μM) . ( B ) Anti-inflammatory activity of pretreatment for 1 h with 1 , 2 (50, 10, 1 and 0.1 μM), or vehicle control (0.5% DMSO) by measuring the production of nitric oxide (NO) in murine macrophages (RAW264.7) 24 h after LPS stimulation. ( C ) <t>Cell</t> <t>viability</t> of RAW264.7 cells using the MTT assay at 24 h under the same conditions used in the NO assay. ( D ) iNOS target gene expression in RAW264.7 after pretreatment for 1 h with 1 or vehicle control (0.5% DMSO prior to LPS addition for 3 and 12 h). RNA was isolated, reverse-transcribed, and subjected to qPCR, using β -actin as the endogenous control. The values were normalized to vehicle control treated with LPS for each time point. Non-stimulated cells (no LPS) were tested simultaneously ( B – D ). Error bars indicate the mean ± SD of three replicates for graphs B, C, and D. Statistical analysis was performed using multiple comparison t-tests (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, compared to LPS treatment alone).
Mtt Dye, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mtt+dye/pmc08911782-99-10-16?v=Promega
Average 90 stars, based on 1 article reviews
mtt dye - by Bioz Stars, 2026-07
90/100 stars
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mtt dye concentration  (HiMedia Laboratories)
90
HiMedia Laboratories mtt dye concentration
Bioactivity of (4 E )- R -dysidazirine carboxylic acid ( 1 ) and methyl ester 2. ( A ) Antiproliferative activity against human colon cancer cells (HCT116) measured by <t>MTT</t> assay at 48 h. Gatorbulin-1 was used as a positive control, tested at the same time (IC 50 0.80 μM) . ( B ) Anti-inflammatory activity of pretreatment for 1 h with 1 , 2 (50, 10, 1 and 0.1 μM), or vehicle control (0.5% DMSO) by measuring the production of nitric oxide (NO) in murine macrophages (RAW264.7) 24 h after LPS stimulation. ( C ) <t>Cell</t> <t>viability</t> of RAW264.7 cells using the MTT assay at 24 h under the same conditions used in the NO assay. ( D ) iNOS target gene expression in RAW264.7 after pretreatment for 1 h with 1 or vehicle control (0.5% DMSO prior to LPS addition for 3 and 12 h). RNA was isolated, reverse-transcribed, and subjected to qPCR, using β -actin as the endogenous control. The values were normalized to vehicle control treated with LPS for each time point. Non-stimulated cells (no LPS) were tested simultaneously ( B – D ). Error bars indicate the mean ± SD of three replicates for graphs B, C, and D. Statistical analysis was performed using multiple comparison t-tests (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, compared to LPS treatment alone).
Mtt Dye Concentration, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mtt+dye/pmc10572761-86-4-9?v=HiMedia+Laboratories
Average 90 stars, based on 1 article reviews
mtt dye concentration - by Bioz Stars, 2026-07
90/100 stars
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dye reagent 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (mtt)  (Carl Roth GmbH)
90
Carl Roth GmbH dye reagent 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (mtt)
Bioactivity of (4 E )- R -dysidazirine carboxylic acid ( 1 ) and methyl ester 2. ( A ) Antiproliferative activity against human colon cancer cells (HCT116) measured by <t>MTT</t> assay at 48 h. Gatorbulin-1 was used as a positive control, tested at the same time (IC 50 0.80 μM) . ( B ) Anti-inflammatory activity of pretreatment for 1 h with 1 , 2 (50, 10, 1 and 0.1 μM), or vehicle control (0.5% DMSO) by measuring the production of nitric oxide (NO) in murine macrophages (RAW264.7) 24 h after LPS stimulation. ( C ) <t>Cell</t> <t>viability</t> of RAW264.7 cells using the MTT assay at 24 h under the same conditions used in the NO assay. ( D ) iNOS target gene expression in RAW264.7 after pretreatment for 1 h with 1 or vehicle control (0.5% DMSO prior to LPS addition for 3 and 12 h). RNA was isolated, reverse-transcribed, and subjected to qPCR, using β -actin as the endogenous control. The values were normalized to vehicle control treated with LPS for each time point. Non-stimulated cells (no LPS) were tested simultaneously ( B – D ). Error bars indicate the mean ± SD of three replicates for graphs B, C, and D. Statistical analysis was performed using multiple comparison t-tests (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, compared to LPS treatment alone).
Dye Reagent 3 [4,5 Dimethylthiazol 2 Yl] 2,5 Diphenyltetrazolium Bromide (Mtt), supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mtt+dye/10__1016_slash_j__chroma__2017__09__062-36-18-24?v=Carl+Roth+GmbH
Average 90 stars, based on 1 article reviews
dye reagent 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (mtt) - by Bioz Stars, 2026-07
90/100 stars
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mtt dye himedia  (HiMedia Laboratories)
90
HiMedia Laboratories mtt dye himedia
Bioactivity of (4 E )- R -dysidazirine carboxylic acid ( 1 ) and methyl ester 2. ( A ) Antiproliferative activity against human colon cancer cells (HCT116) measured by <t>MTT</t> assay at 48 h. Gatorbulin-1 was used as a positive control, tested at the same time (IC 50 0.80 μM) . ( B ) Anti-inflammatory activity of pretreatment for 1 h with 1 , 2 (50, 10, 1 and 0.1 μM), or vehicle control (0.5% DMSO) by measuring the production of nitric oxide (NO) in murine macrophages (RAW264.7) 24 h after LPS stimulation. ( C ) <t>Cell</t> <t>viability</t> of RAW264.7 cells using the MTT assay at 24 h under the same conditions used in the NO assay. ( D ) iNOS target gene expression in RAW264.7 after pretreatment for 1 h with 1 or vehicle control (0.5% DMSO prior to LPS addition for 3 and 12 h). RNA was isolated, reverse-transcribed, and subjected to qPCR, using β -actin as the endogenous control. The values were normalized to vehicle control treated with LPS for each time point. Non-stimulated cells (no LPS) were tested simultaneously ( B – D ). Error bars indicate the mean ± SD of three replicates for graphs B, C, and D. Statistical analysis was performed using multiple comparison t-tests (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, compared to LPS treatment alone).
Mtt Dye Himedia, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mtt+dye/pmc11484510-280-0-2?v=HiMedia+Laboratories
Average 90 stars, based on 1 article reviews
mtt dye himedia - by Bioz Stars, 2026-07
90/100 stars
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mtt dye  (Beijing Solarbio Science)
90
Beijing Solarbio Science mtt dye
Bioactivity of (4 E )- R -dysidazirine carboxylic acid ( 1 ) and methyl ester 2. ( A ) Antiproliferative activity against human colon cancer cells (HCT116) measured by <t>MTT</t> assay at 48 h. Gatorbulin-1 was used as a positive control, tested at the same time (IC 50 0.80 μM) . ( B ) Anti-inflammatory activity of pretreatment for 1 h with 1 , 2 (50, 10, 1 and 0.1 μM), or vehicle control (0.5% DMSO) by measuring the production of nitric oxide (NO) in murine macrophages (RAW264.7) 24 h after LPS stimulation. ( C ) <t>Cell</t> <t>viability</t> of RAW264.7 cells using the MTT assay at 24 h under the same conditions used in the NO assay. ( D ) iNOS target gene expression in RAW264.7 after pretreatment for 1 h with 1 or vehicle control (0.5% DMSO prior to LPS addition for 3 and 12 h). RNA was isolated, reverse-transcribed, and subjected to qPCR, using β -actin as the endogenous control. The values were normalized to vehicle control treated with LPS for each time point. Non-stimulated cells (no LPS) were tested simultaneously ( B – D ). Error bars indicate the mean ± SD of three replicates for graphs B, C, and D. Statistical analysis was performed using multiple comparison t-tests (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, compared to LPS treatment alone).
Mtt Dye, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mtt+dye/pm36411664-59-1-13?v=Beijing+Solarbio+Science
Average 90 stars, based on 1 article reviews
mtt dye - by Bioz Stars, 2026-07
90/100 stars
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mtt dye  (Arkat USA Inc)
90
Arkat USA Inc mtt dye
Bioactivity of (4 E )- R -dysidazirine carboxylic acid ( 1 ) and methyl ester 2. ( A ) Antiproliferative activity against human colon cancer cells (HCT116) measured by <t>MTT</t> assay at 48 h. Gatorbulin-1 was used as a positive control, tested at the same time (IC 50 0.80 μM) . ( B ) Anti-inflammatory activity of pretreatment for 1 h with 1 , 2 (50, 10, 1 and 0.1 μM), or vehicle control (0.5% DMSO) by measuring the production of nitric oxide (NO) in murine macrophages (RAW264.7) 24 h after LPS stimulation. ( C ) <t>Cell</t> <t>viability</t> of RAW264.7 cells using the MTT assay at 24 h under the same conditions used in the NO assay. ( D ) iNOS target gene expression in RAW264.7 after pretreatment for 1 h with 1 or vehicle control (0.5% DMSO prior to LPS addition for 3 and 12 h). RNA was isolated, reverse-transcribed, and subjected to qPCR, using β -actin as the endogenous control. The values were normalized to vehicle control treated with LPS for each time point. Non-stimulated cells (no LPS) were tested simultaneously ( B – D ). Error bars indicate the mean ± SD of three replicates for graphs B, C, and D. Statistical analysis was performed using multiple comparison t-tests (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, compared to LPS treatment alone).
Mtt Dye, supplied by Arkat USA Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mtt+dye/10__24820_slash_ark__5550190__p010__472-61-9-3?v=Arkat+USA+Inc
Average 90 stars, based on 1 article reviews
mtt dye - by Bioz Stars, 2026-07
90/100 stars
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tetrazolium dye (mtt)  (Sinopharm ltd)
90
Sinopharm ltd tetrazolium dye (mtt)
Bioactivity of (4 E )- R -dysidazirine carboxylic acid ( 1 ) and methyl ester 2. ( A ) Antiproliferative activity against human colon cancer cells (HCT116) measured by <t>MTT</t> assay at 48 h. Gatorbulin-1 was used as a positive control, tested at the same time (IC 50 0.80 μM) . ( B ) Anti-inflammatory activity of pretreatment for 1 h with 1 , 2 (50, 10, 1 and 0.1 μM), or vehicle control (0.5% DMSO) by measuring the production of nitric oxide (NO) in murine macrophages (RAW264.7) 24 h after LPS stimulation. ( C ) <t>Cell</t> <t>viability</t> of RAW264.7 cells using the MTT assay at 24 h under the same conditions used in the NO assay. ( D ) iNOS target gene expression in RAW264.7 after pretreatment for 1 h with 1 or vehicle control (0.5% DMSO prior to LPS addition for 3 and 12 h). RNA was isolated, reverse-transcribed, and subjected to qPCR, using β -actin as the endogenous control. The values were normalized to vehicle control treated with LPS for each time point. Non-stimulated cells (no LPS) were tested simultaneously ( B – D ). Error bars indicate the mean ± SD of three replicates for graphs B, C, and D. Statistical analysis was performed using multiple comparison t-tests (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, compared to LPS treatment alone).
Tetrazolium Dye (Mtt), supplied by Sinopharm ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mtt+dye/pmc12138606-56-69-75?v=Sinopharm+ltd
Average 90 stars, based on 1 article reviews
tetrazolium dye (mtt) - by Bioz Stars, 2026-07
90/100 stars
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mtt dye solution  (Promega)
90
Promega mtt dye solution
Bioactivity of (4 E )- R -dysidazirine carboxylic acid ( 1 ) and methyl ester 2. ( A ) Antiproliferative activity against human colon cancer cells (HCT116) measured by <t>MTT</t> assay at 48 h. Gatorbulin-1 was used as a positive control, tested at the same time (IC 50 0.80 μM) . ( B ) Anti-inflammatory activity of pretreatment for 1 h with 1 , 2 (50, 10, 1 and 0.1 μM), or vehicle control (0.5% DMSO) by measuring the production of nitric oxide (NO) in murine macrophages (RAW264.7) 24 h after LPS stimulation. ( C ) <t>Cell</t> <t>viability</t> of RAW264.7 cells using the MTT assay at 24 h under the same conditions used in the NO assay. ( D ) iNOS target gene expression in RAW264.7 after pretreatment for 1 h with 1 or vehicle control (0.5% DMSO prior to LPS addition for 3 and 12 h). RNA was isolated, reverse-transcribed, and subjected to qPCR, using β -actin as the endogenous control. The values were normalized to vehicle control treated with LPS for each time point. Non-stimulated cells (no LPS) were tested simultaneously ( B – D ). Error bars indicate the mean ± SD of three replicates for graphs B, C, and D. Statistical analysis was performed using multiple comparison t-tests (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, compared to LPS treatment alone).
Mtt Dye Solution, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mtt+dye/pm28542418-73-3-6?v=Promega
Average 90 stars, based on 1 article reviews
mtt dye solution - by Bioz Stars, 2026-07
90/100 stars
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mtt dye  (Biomatik)
90
Biomatik mtt dye
Bioactivity of (4 E )- R -dysidazirine carboxylic acid ( 1 ) and methyl ester 2. ( A ) Antiproliferative activity against human colon cancer cells (HCT116) measured by <t>MTT</t> assay at 48 h. Gatorbulin-1 was used as a positive control, tested at the same time (IC 50 0.80 μM) . ( B ) Anti-inflammatory activity of pretreatment for 1 h with 1 , 2 (50, 10, 1 and 0.1 μM), or vehicle control (0.5% DMSO) by measuring the production of nitric oxide (NO) in murine macrophages (RAW264.7) 24 h after LPS stimulation. ( C ) <t>Cell</t> <t>viability</t> of RAW264.7 cells using the MTT assay at 24 h under the same conditions used in the NO assay. ( D ) iNOS target gene expression in RAW264.7 after pretreatment for 1 h with 1 or vehicle control (0.5% DMSO prior to LPS addition for 3 and 12 h). RNA was isolated, reverse-transcribed, and subjected to qPCR, using β -actin as the endogenous control. The values were normalized to vehicle control treated with LPS for each time point. Non-stimulated cells (no LPS) were tested simultaneously ( B – D ). Error bars indicate the mean ± SD of three replicates for graphs B, C, and D. Statistical analysis was performed using multiple comparison t-tests (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, compared to LPS treatment alone).
Mtt Dye, supplied by Biomatik, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mtt+dye/pm35163281-121-71-47?v=Biomatik
Average 90 stars, based on 1 article reviews
mtt dye - by Bioz Stars, 2026-07
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tetrazolium (mtt) dye assay  (FUJIFILM)
90
FUJIFILM tetrazolium (mtt) dye assay
Bioactivity of (4 E )- R -dysidazirine carboxylic acid ( 1 ) and methyl ester 2. ( A ) Antiproliferative activity against human colon cancer cells (HCT116) measured by <t>MTT</t> assay at 48 h. Gatorbulin-1 was used as a positive control, tested at the same time (IC 50 0.80 μM) . ( B ) Anti-inflammatory activity of pretreatment for 1 h with 1 , 2 (50, 10, 1 and 0.1 μM), or vehicle control (0.5% DMSO) by measuring the production of nitric oxide (NO) in murine macrophages (RAW264.7) 24 h after LPS stimulation. ( C ) <t>Cell</t> <t>viability</t> of RAW264.7 cells using the MTT assay at 24 h under the same conditions used in the NO assay. ( D ) iNOS target gene expression in RAW264.7 after pretreatment for 1 h with 1 or vehicle control (0.5% DMSO prior to LPS addition for 3 and 12 h). RNA was isolated, reverse-transcribed, and subjected to qPCR, using β -actin as the endogenous control. The values were normalized to vehicle control treated with LPS for each time point. Non-stimulated cells (no LPS) were tested simultaneously ( B – D ). Error bars indicate the mean ± SD of three replicates for graphs B, C, and D. Statistical analysis was performed using multiple comparison t-tests (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, compared to LPS treatment alone).
Tetrazolium (Mtt) Dye Assay, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mtt+dye/pmc09323155-158-6-10?v=FUJIFILM
Average 90 stars, based on 1 article reviews
tetrazolium (mtt) dye assay - by Bioz Stars, 2026-07
90/100 stars
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mtt dye  (MultiSciences Biotech Co Ltd)
90
MultiSciences Biotech Co Ltd mtt dye
Bioactivity of (4 E )- R -dysidazirine carboxylic acid ( 1 ) and methyl ester 2. ( A ) Antiproliferative activity against human colon cancer cells (HCT116) measured by <t>MTT</t> assay at 48 h. Gatorbulin-1 was used as a positive control, tested at the same time (IC 50 0.80 μM) . ( B ) Anti-inflammatory activity of pretreatment for 1 h with 1 , 2 (50, 10, 1 and 0.1 μM), or vehicle control (0.5% DMSO) by measuring the production of nitric oxide (NO) in murine macrophages (RAW264.7) 24 h after LPS stimulation. ( C ) <t>Cell</t> <t>viability</t> of RAW264.7 cells using the MTT assay at 24 h under the same conditions used in the NO assay. ( D ) iNOS target gene expression in RAW264.7 after pretreatment for 1 h with 1 or vehicle control (0.5% DMSO prior to LPS addition for 3 and 12 h). RNA was isolated, reverse-transcribed, and subjected to qPCR, using β -actin as the endogenous control. The values were normalized to vehicle control treated with LPS for each time point. Non-stimulated cells (no LPS) were tested simultaneously ( B – D ). Error bars indicate the mean ± SD of three replicates for graphs B, C, and D. Statistical analysis was performed using multiple comparison t-tests (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, compared to LPS treatment alone).
Mtt Dye, supplied by MultiSciences Biotech Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mtt+dye/pm28245474-71-10-16?v=MultiSciences+Biotech+Co+Ltd
Average 90 stars, based on 1 article reviews
mtt dye - by Bioz Stars, 2026-07
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mitochondrial dye conversion assay mtt  (Promega)
90
Promega mitochondrial dye conversion assay mtt
Bioactivity of (4 E )- R -dysidazirine carboxylic acid ( 1 ) and methyl ester 2. ( A ) Antiproliferative activity against human colon cancer cells (HCT116) measured by <t>MTT</t> assay at 48 h. Gatorbulin-1 was used as a positive control, tested at the same time (IC 50 0.80 μM) . ( B ) Anti-inflammatory activity of pretreatment for 1 h with 1 , 2 (50, 10, 1 and 0.1 μM), or vehicle control (0.5% DMSO) by measuring the production of nitric oxide (NO) in murine macrophages (RAW264.7) 24 h after LPS stimulation. ( C ) <t>Cell</t> <t>viability</t> of RAW264.7 cells using the MTT assay at 24 h under the same conditions used in the NO assay. ( D ) iNOS target gene expression in RAW264.7 after pretreatment for 1 h with 1 or vehicle control (0.5% DMSO prior to LPS addition for 3 and 12 h). RNA was isolated, reverse-transcribed, and subjected to qPCR, using β -actin as the endogenous control. The values were normalized to vehicle control treated with LPS for each time point. Non-stimulated cells (no LPS) were tested simultaneously ( B – D ). Error bars indicate the mean ± SD of three replicates for graphs B, C, and D. Statistical analysis was performed using multiple comparison t-tests (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, compared to LPS treatment alone).
Mitochondrial Dye Conversion Assay Mtt, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mtt+dye/pmc02014778-270-9-17?v=Promega
Average 90 stars, based on 1 article reviews
mitochondrial dye conversion assay mtt - by Bioz Stars, 2026-07
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Image Search Results


Bioactivity of (4 E )- R -dysidazirine carboxylic acid ( 1 ) and methyl ester 2. ( A ) Antiproliferative activity against human colon cancer cells (HCT116) measured by MTT assay at 48 h. Gatorbulin-1 was used as a positive control, tested at the same time (IC 50 0.80 μM) . ( B ) Anti-inflammatory activity of pretreatment for 1 h with 1 , 2 (50, 10, 1 and 0.1 μM), or vehicle control (0.5% DMSO) by measuring the production of nitric oxide (NO) in murine macrophages (RAW264.7) 24 h after LPS stimulation. ( C ) Cell viability of RAW264.7 cells using the MTT assay at 24 h under the same conditions used in the NO assay. ( D ) iNOS target gene expression in RAW264.7 after pretreatment for 1 h with 1 or vehicle control (0.5% DMSO prior to LPS addition for 3 and 12 h). RNA was isolated, reverse-transcribed, and subjected to qPCR, using β -actin as the endogenous control. The values were normalized to vehicle control treated with LPS for each time point. Non-stimulated cells (no LPS) were tested simultaneously ( B – D ). Error bars indicate the mean ± SD of three replicates for graphs B, C, and D. Statistical analysis was performed using multiple comparison t-tests (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, compared to LPS treatment alone).

Journal: Molecules

Article Title: Anti-Inflammatory Dysidazirine Carboxylic Acid from the Marine Cyanobacterium Caldora sp. Collected from the Reefs of Fort Lauderdale, Florida †

doi: 10.3390/molecules27051717

Figure Lengend Snippet: Bioactivity of (4 E )- R -dysidazirine carboxylic acid ( 1 ) and methyl ester 2. ( A ) Antiproliferative activity against human colon cancer cells (HCT116) measured by MTT assay at 48 h. Gatorbulin-1 was used as a positive control, tested at the same time (IC 50 0.80 μM) . ( B ) Anti-inflammatory activity of pretreatment for 1 h with 1 , 2 (50, 10, 1 and 0.1 μM), or vehicle control (0.5% DMSO) by measuring the production of nitric oxide (NO) in murine macrophages (RAW264.7) 24 h after LPS stimulation. ( C ) Cell viability of RAW264.7 cells using the MTT assay at 24 h under the same conditions used in the NO assay. ( D ) iNOS target gene expression in RAW264.7 after pretreatment for 1 h with 1 or vehicle control (0.5% DMSO prior to LPS addition for 3 and 12 h). RNA was isolated, reverse-transcribed, and subjected to qPCR, using β -actin as the endogenous control. The values were normalized to vehicle control treated with LPS for each time point. Non-stimulated cells (no LPS) were tested simultaneously ( B – D ). Error bars indicate the mean ± SD of three replicates for graphs B, C, and D. Statistical analysis was performed using multiple comparison t-tests (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, compared to LPS treatment alone).

Article Snippet: Cell viability was measured after 48 h, following treatment with MTT dye using the manufacturer’s protocol (Promega, Madison, WI, USA).

Techniques: Activity Assay, MTT Assay, Positive Control, Control, Targeted Gene Expression, Isolation, Reverse Transcription, Comparison